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Fig. 6 | Plant Methods

Fig. 6

From: Hydroponic cultivation conditions allowing the reproducible investigation of poplar root suberization and water transport

Fig. 6

Results of the root transport physiology experiments. Measurements were taken in the absence and presence of applied pneumatic pressure using a pressure chamber (a–d) or pressure probe (d). The roots investigated were cultivated for 5 weeks in hydroponic control conditions. Xylem sap exudates were plotted against the time to allow the calculation of the volume flow (a). When the volume flow normalized to the root surface area was plotted as a function of the applied pneumatic pressure (b), linear slopes (a line is drawn to guide the eye) were identified in between 0.2 and 0.4 MPa and used to calculate the corresponding hydrostatic hydraulic conductivity Lpr(HY). When the volume flow per unit surface area of the root system is drawn as a function of either P or (P + Δπ σsr) (c), reduced importance of the osmotic term (Δπ σsr) with higher applied pneumatic pressures (P) due to dilution and filtration effects can be observed. The calculated Lpr(HY) is two- to tenfold higher than the osmotic hydraulic conductivity Lpr(OS), depending on the method used (d). Means with or without standard deviations (n = 10 individual stem cutting root systems or 6 individual whole roots) are shown

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