Fig. 2
Comparison between methods for quantification of Pst::LUX colonisation in Arabidopsis plants varying in SA-dependent resistance. A Shown are mean Log10-transformed colony forming units (CFU) per milligram of fresh weight (black bars) and mean relative bioluminescence values per plant (white bars) for hyper-susceptible NahG plants, susceptible Col-0 plants and resistant Col-0 plants that had been pretreated with 250 μM BTH. Bacterial bioluminescence was measured in planta before samples were harvested for colony plating. Different letters indicate statistically significant differences between genotypes/treatments, using one-way ANOVA, followed by Tukey’s post-hoc analysis for multiple comparisons (p < 0.05; small letters: plate dilution analysis; capital letters: relative bioluminescence analysis). Error bars represent standard errors of the mean (n = 24). B Pearson’s correlation analysisb etween CFUs per milligram of plant fresh weight and relative bioluminescence. Dots represent individual samples from all genotypes/treatment combinations presented in A