Table 2 Primer pairs obtained from SIGMA were tested by conventional endpoint PCR to confirm a single product of the correct size was amplified
Reference gene Sequence and source | Primer name | Sequence (5’-3’) and melting temperature (Tm) | Amplicon |
|---|---|---|---|
Leptosphaeria maculans Hypothetical Protein (LEMA_P025150.1) | LmHypPro-F | GCGCGAATCACCAGATACA (Tm = 58.0 °C) | 124 bp |
LmHypPro-R | CTCCTCTAGGGAAGGACATACA (Tm = 57.8 °C) | ||
LmHypPro-Pr | 5’-/56-FAM/TGCCGCGCT/ZEN/GGTATAGTCCGAT/3IABkFQ/-3’ (Tm = 66.1 °C) | ||
Brassica napus High Mobility Group Protein (NM_001316094.1) (Petrie et al. [29]; Weng et al. [34, 35]) | BnHMG-F | GCGAAGCACATCGAGTCA (Tm = 57.8 °C) | 73 bp |
BnHMG-R | GGTTGAGGTGGTAGCTGAGG (Tm = 59.7 °C) | ||
BnHMG-Pr | 5’-/5HEX/TCTCTACCA/ZEN/CCGTCTCACATGACGC/3IABkFQ/-3’ (Tm = 66.0 °C) | ||