Skip to main content
Fig. 3 | Plant Methods

Fig. 3

From: Comparison of non-subjective relative fungal biomass measurements to quantify the Leptosphaeria maculans—Brassica napus interaction

Fig. 3

L. maculans fungal biomass assays of, and progression, in stems (Experiment 2). WAC assays (A, B) and ddPCR assays (C, D) of the crown (A, C) at the cotyledon petiole-stem junction and hypocotyl (B, D) directly below the crown. WAC and ddPCR results revealed similar trends in WAC and ddPCR for both crown (A, C) and the hypocotyl sections (B, D). WAC (diagrams A, B) y-axes scales are presented as ratios of cultivar RFU means/mock background RFU means. Standard error bars in WAC assays were calculated based on at least three technical replicates for each of 14 biological replicates. Standard error bars in ddPCR assays (C, D) were calculated based on 14 biological replicates. (ATR-Mako, ATR-Bonito, and Hyola350TT cultivars are referred to as Mako, Bonito and Hyola, respectively). P < 0.02 for all timepoint data sets except for 4 wpi (T3) ddPCR data set C where P < 0.16

Back to article page

Plant Methods

ISSN: 1746-4811