Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants

Table 1 T-DNA vectors for fluorescent seed selection

Destination vector	Addgene no.	Gateway adapters	Promoter::Cas	Plant selection	Bacterial selection
pRU051	167677	attR1–attR2	PcUBi4-2::SpCas9	FastRed	Spectinomycin
pRU052	167678	attR1–attR2	PcUBi4-2::SpCas9	FastGreen	Spectinomycin
pRU053	167679	attR1–attR2	pEC1.2::SpCas9	FastRed	Spectinomycin
pRU054	167680	attR1–attR2	pEC1.2::SpCas9	FastGreen	Spectinomycin
pRU321	167681	attR1–attR2	PcUBi4-2::SaCas9	FastRed	Spectinomycin
pRU320	167682	attR1–attR2	PcUBi4-2::SaCas9	FastGreen	Spectinomycin
pRU322	167683	attR1–attR2	pEC1.2::SaCas9	FastGreen	Spectinomycin
pRU055	167684	attR1–attR2	pEC1.2::AsCas12a^a	FastRed	Spectinomycin
pRU205	167685	attR1–attR2	PcUBi4-2::AsCas12a	FastGreen	Spectinomycin
pRU319	167686	attR1–attR2	pEC1.2::SpCas9^a	FastRed	Spectinomycin
pRU292	167687	attR1–attR2	PcUBi4-2::zCas9i	FastRed	Spectinomycin
pRU293	167688	attR1–attR2	pUBi4-2::zCas9i	FastGreen	Spectinomycin
pRU294	167689	attR1–attR2	pEC1.2::zCas9i	FastRed	Spectinomycin
pRU323	167690	attR1–attR2	PcUBi4-2::zCas9i	FastCyan	Spectinomycin
pRU324	167691	attR1–attR2	pEC1.2::zCas9i	FastCyan	Spectinomycin
pRU061	167692	attR1–attR2	PcUBi4-2::AsCas12a	FastRed	Spectinomycin
pRU206	167693	attR1–attR2	pEC1.2::AsCas12a	FastGreen	Spectinomycin
pRU295	167694	attR1–attR2	pEC1.2::zCas9i	FastGreen	Spectinomycin

ISSN: 1746-4811