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Fig. 3 | Plant Methods

Fig. 3

From: Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants

Fig. 3

Example of the application of co-dipping strategy. a Graphical representation of CUC1 and CUC2 genes and positions of gRNAs. b cuc1cuc2 knockout cotyledon phenotype in the isolated pEC1.2::Cas9 (T2-5) and PcUBi4-2::Cas9 (T2-18) lines. c Segregation analysis of the selected T2 lines. Counting the non-fluorescent seeds and evaluating cuc1cuc2 phenotype among the non-fluorescent seeds were performed independently. d Segregation analysis in 36 wild-type looking individuals derived from non-fluorescent seeds from the selected pEC1.2::Cas9 (T2-5) and PcUBi4-2::Cas9 (T2-18) lines. Yellow color indicates homozygous, green indicates heterozygous and grey color indicates wild-type individuals. The asterisks indicate the individuals which are homozygous for one of the two genes and heterozygous for the other one. e The chromatograms showing the type of mutations in pEC1.2::Cas9 (T2-5) and PcUBi4-2::Cas9 (T2-18) lines as identified by sequencing. Scale bar in b  =  1 mm

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Plant Methods

ISSN: 1746-4811