Fig. 4

Integrated analysis on the datasets reveals distinct morphological diversity of ER bodies between A. thaliana wild type and mutants. A The heatmap represents the z-score measure of the 40 morphological parameters (x-axis) and segmented cells clustered to show ER body phenotype. These clustered cells belong to their independent experiments taking in 2 different settings (Setting 1 and Setting 2) and 2 objectives (20× and 25×). B Constrained ordination analysis (CCA) was performed on the z-scores of the morphological parameters of the clustered cells for each of the ER bodies detected from the independent experiments, using genotype as a fixed factor. The difference in the colour of the dotted circle represents the genotypic differences. The variance explained by genotype is 1.37%. C CCA was performed on aggregated morphological parameters of the clustered cells. The variance explained by genotype is above 7%. Note that the dispersions between the leb-1 bglu21-1 (blue-dotted circle), meb2-1 (dark yellow-dotted circle) and meb1-1 meb2-1 (light green-dotted circle) are distinct from the wild type (purple-dotted circle) in the scatterplot. D The heatmap represents a confusion matrix from discriminant analysis performed by using the FDA and MDA methods