Fig. 7

Difference in UFGT fold-change (FC) called in datasets normalized to CYSP and to NAD5 in GLRaV-3-infected leaf at E-L 35 (left), and GLRaV-3-infected berry at E-L 38 (right). Statistical difference between the FC values called in CYSP-dataset and in NAD5-dataset was analyzed using one-way ANOVA. In GLRaV-3-infected leaf (left), UFGT was up-regulated by 1.44-folds when dataset was normalized against CYSP and by 2.50-folds when normalized against NAD5. The FC value from NAD5-dataset was significantly different from that from CYSP-dataset (***P = 0). In GLRaV-3-infected berry at E-L 38 (right), UFGT was down-regulated by − 1.33-folds when dataset was normalized against CYSP and by − 1.62-folds when dataset was normalized against NAD5. No significant difference was found between the two FC values (ns, P = 0.23303). UFGT fold change values were calculated using Pfaffl method [95]. FC values are mean, ± SD (error bars), n = 3. Positive FC values represent up-regulation of gene (in response to GLRaV-3 infection) and are depicted by red arrows. Negative FC values represent down-regulation (in response to GLRaV-3 infection) and are depicted by green arrows. ns: not significant (P > 0.05); *significant (0.01 < P < 0.05); **significant (0.001 < P < 0.01); ***significant (P < 0.001)