Fig. 6

UFGT relative expressions as normalized against three reference genes (CYSP, actin, and NAD5), in GLRaV-3-infected (orange bars) as compared to GLRaV-3-free (grey bars) E-L 35 leaf (A) and E-L 38 berry (B) of Cabernet Franc. Statistical significance of UFGT expressional change in GLRaV-3-infected vs. GLRaV-3-free samples was evaluated using one-way ANOVA. A In Cabernet Franc leaf at E-L 35, dataset normalized to CYSP (left, **P = 0.00768) and to NAD5 (right, ***P = 0.00079) showed that UFGT expression in GLRaV-3-infected leaf was significantly different from that in GLRaV-3-free leaf. Data set normalized to actin (middle) showed no significant difference between GLRaV-3-infected leaf and GLRaV-3-free leaf (ns, P = 0.23146). B In berry at E-L38, data sets normalized to CYSP (left, *P = 0.02235) and to NAD5 (right, *P = 0.03925) showed that UFGT expression was significantly different in GLRaV-3-infected samples compared to GLRaV-3-free samples. However, data set normalized to actin (middle) revealed no significant difference in UFGT expression due to GLRaV-3-infection (ns, P = 0.77989). UFGT relative expressions were generated by normalizing raw quantification data against each of the three reference genes (CYSP, actin, NAD5). Relative expression values are mean, ± SD (error bars), n = 3. ns: not significant (P > 0.05); *significant (0.01 < P < 0.05); **significant (0.001 < P < 0.01); ***significant (P < 0.001)