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Fig. 5 | Plant Methods

Fig. 5

From: Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries

Fig. 5

Expression profile of reference gene candidates of grapevine samples. For each reference gene candidate, absolute Ct values from all sample groups were combined. Sample groups comprised two tissue types (leaf and berry) at three developmental stages (E-L 31, E-L 35 and E-L 38), infected with and free from GLRaV-3. Each sample group has three biological replicates. The boxes indicate the range of scores between the 25th and the 75th percentiles. The thick line within each box represents the median. Maximum and minimum values are denoted by whiskers. Small circles represent outliers. Reference gene candidates with the highest CV (actin) and the lowest CV (Gluc) are highlighted in bold

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