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Fig. 4 | Plant Methods

Fig. 4

From: Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries

Fig. 4

RT-PCR of Grapevine leafroll-associated virus 3 (GLRaV-3) and grapevine phytoene desaturase synthase (PDS) using total RNAs extracted from leaf and berry samples of dark-skinned wine grape cultivars. A Detection of GLRaV-3 in leaf and berry samples of Cabernet Franc. Lanes 1–3: samples free of GLRaV-3; lanes 4–6: samples infected with GLRaV-3; B detection of PDS from mature berries collected from nine dark-skinned wine grape varieties at E-L 38. Two samples for each cultivar were included in the test. Fainter bands for berry samples in lane 6 of each set in Fig. 4A were due to lower virus titer in this vine. M.F. = Marechal Foch; P.N. = Pinot Noir; P.M. = Pinot Meunier; GA = Gamay; C.S = Cabernet. Sauvignon; D.C. = De Chaunac, Mer = Merlot; Cham = Chambourcin; B.N. = Baco Noir; NC = negative control

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Plant Methods

ISSN: 1746-4811