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Fig. 6 | Plant Methods

Fig. 6

From: A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Fig. 6

Dual-LUC assay in A. annua cells. a Activation of CYP71AV1 gene promoter by AaGSW1 and AaORA. b Activation of AaHD1, AaLTP1 and AaLTP2 gene promoters by AaHD8. The pHB-YFP was used as a negative control. The promoters of AaCYP71AVA, AaHD1, AaLTP1 and AaLTP2 were fused to drive the expression of Firefly luciferase (LUC) gene while the Renilla luciferase (REN) gene under the control of a CaMV35S promoter was used as an internal control, and the LUC/REN ratio of YFP was set as 1. Error bars indicate SD (n = 3). Student’s t-test: **, P < 0.01

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