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Fig. 3 | Plant Methods

Fig. 3

From: A high-efficiency Agrobacterium-mediated transient expression system in the leaves of Artemisia annua L.

Fig. 3

Development of transient expression system in A. annua leaves. a Leaves infiltrated with Agrobacterium strain cells (LBA4404, GV30101, EHA105) carrying pEAQ-HT-DEST1-eGFP construct. b, c Intensity of LUC bioluminescence quantified using GloMax® 20/20 Luminometer (Promega), ck: without leaf-injection, ns: no surfactant. d Leaves infiltrated with Agrobacterium strain EHA105 cells harboring pEAQ-HT-DEST1-eGFP construct using optimized transformation method. e Western blot analysis of leaves infiltrated with Agrobacterium strain EHA105 cells harboring pEAQ-HT-eGFP construct (+) or no injection (−). Fluorescence microscopy was performed at 3 dpi and images were collected using the 4 × and 10 × objective. Error bars indicate SD (n = 3). Student’s t-test: **P < 0.01

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Plant Methods

ISSN: 1746-4811