Fig. 3
From: A high throughput assay of lichenase activity with Congo red dye in plants
Fluorometric assessment of thermostable lichenase activity using Congo red. a Electrophoretic pattern of bacterial protein samples with thermostable lichenase before and after affinity chromatography purification; M, molecular weight marker (Thermo Scientific PageRuler Unstained Protein Ladder (Thermo Fisher Scientific, Inc., Waltham, Massachusetts, United States). b Comparative analysis of the fluorescence intensity of CR–lichenan complex depending on the lichenase amount in preparation and the time of incubation with the substrate (incubation time vector, 5, 10, 20, 40, 60, 120, and 180 min; protein (lichenase) amount vector, 16, 8, 4, 2, 1. 0.5, 0.25, 0.125, 0.0625, and 0.03125 μg/sample)