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Table 1 Identification of selected protein spots from K. obovata leaves by MALDI-TOF/TOF-MS

From: An efficient protein extraction method applied to mangrove plant Kandelia obovata leaves for proteomic analysis

Spot no.

Genbank accession

Protein identification

Species

Mr (kDa)

pI

Score

Expect

Matches

Sequence coverage (%)

Pred.

Obs.

Pred.

Obs.

1

gi|672134785

ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic

Phoenix dactylifera

102

95

6.13

5.65

554

9.00E-50

32

33

2

gi|255566397

aconitase, putative

Ricinus communis

109.4

97

7.11

5.9

203

1.10E-14

15

15

3a

gi|394831087

ATP synthase CF1 alpha subunit (chloroplast)

Datura stramonium

55.49

54

5.15

5.68

545

1.50E-47

18

30

4a

gi|297804102

Chloroplast elongation factor tub

Arabidopsis lyrata subsp. lyrata

51.87

49

5.84

5.62

267

4.50E-21

8

19

5

gi|659087282

Glutamate--glyoxylate aminotransferase 2

Cucumis melo

52.95

51

5.63

6.02

319

2.90E-26

15

28

6a

gi|571475731

Putative lactoylglutathione lyase-like isoform X3

Glycine max

32.5

31

5.76

5.21

210

2.30E-15

5

13

7

gi|645277823

Adenosine kinase 2

Prunus mume

37.96

37

5.22

5.22

248

3.60E-19

7

24

8

gi|568214952

Fructose-1,6-bisphosphatase, cytosolic

Solanum tuberosum

37.74

38

5.8

5.98

194

9.00E-14

9

27

9

gi|255566888

Plastid-specific 30S ribosomal protein 1, chloroplast precursor, putative

Ricinus communis

35.02

36

9.18

6.15

259

2.90E-20

7

18

10

gi|672158791

Thioredoxin reductase NTRB-like

Phoenix dactylifera

35.63

35

5.92

6

83

0.012

4

10

11

gi|8131593

Oxygen evolving enhancer protein 2

Bruguiera gymnorhiza

17.58

17

4.91

5.3

181

1.80E-12

5

40

12

gi|657998060

Proteasome subunit beta type-6-like

Malus domestica

25.84

24

5.03

5.4

220

2.30E-16

9

33

13

gi|22797141

ATP synthase epsilon subunit

Olea europaea

14.68

15

6.51

5.08

128

3.60E-07

4

39

14

gi|587906415

Elongation factor Tu

Morus notabilis

52.23

51

6.3

5.05

109

2.90E-05

5

16

  1. Predicted (Pred.) and observed (Obs.) Mr and pI values and sequence coverage are shown
  2. aPeptide spot that is consistent in different extracts