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Table 1 Identification of selected protein spots from K. obovata leaves by MALDI-TOF/TOF-MS

From: An efficient protein extraction method applied to mangrove plant Kandelia obovata leaves for proteomic analysis

Spot no. Genbank accession Protein identification Species Mr (kDa) pI Score Expect Matches Sequence coverage (%)
Pred. Obs. Pred. Obs.
1 gi|672134785 ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic Phoenix dactylifera 102 95 6.13 5.65 554 9.00E-50 32 33
2 gi|255566397 aconitase, putative Ricinus communis 109.4 97 7.11 5.9 203 1.10E-14 15 15
3a gi|394831087 ATP synthase CF1 alpha subunit (chloroplast) Datura stramonium 55.49 54 5.15 5.68 545 1.50E-47 18 30
4a gi|297804102 Chloroplast elongation factor tub Arabidopsis lyrata subsp. lyrata 51.87 49 5.84 5.62 267 4.50E-21 8 19
5 gi|659087282 Glutamate--glyoxylate aminotransferase 2 Cucumis melo 52.95 51 5.63 6.02 319 2.90E-26 15 28
6a gi|571475731 Putative lactoylglutathione lyase-like isoform X3 Glycine max 32.5 31 5.76 5.21 210 2.30E-15 5 13
7 gi|645277823 Adenosine kinase 2 Prunus mume 37.96 37 5.22 5.22 248 3.60E-19 7 24
8 gi|568214952 Fructose-1,6-bisphosphatase, cytosolic Solanum tuberosum 37.74 38 5.8 5.98 194 9.00E-14 9 27
9 gi|255566888 Plastid-specific 30S ribosomal protein 1, chloroplast precursor, putative Ricinus communis 35.02 36 9.18 6.15 259 2.90E-20 7 18
10 gi|672158791 Thioredoxin reductase NTRB-like Phoenix dactylifera 35.63 35 5.92 6 83 0.012 4 10
11 gi|8131593 Oxygen evolving enhancer protein 2 Bruguiera gymnorhiza 17.58 17 4.91 5.3 181 1.80E-12 5 40
12 gi|657998060 Proteasome subunit beta type-6-like Malus domestica 25.84 24 5.03 5.4 220 2.30E-16 9 33
13 gi|22797141 ATP synthase epsilon subunit Olea europaea 14.68 15 6.51 5.08 128 3.60E-07 4 39
14 gi|587906415 Elongation factor Tu Morus notabilis 52.23 51 6.3 5.05 109 2.90E-05 5 16
  1. Predicted (Pred.) and observed (Obs.) Mr and pI values and sequence coverage are shown
  2. aPeptide spot that is consistent in different extracts