Skip to main content
Fig. 2 | Plant Methods

Fig. 2

From: An efficient protein extraction method applied to mangrove plant Kandelia obovata leaves for proteomic analysis

Fig. 2

Representative SDS-PAGE and 2-DE gels of the total proteins extracted by three methods. (A) SDS-PAGE gel of three protein extracts and protein marker. The black arrow indicated the location of the Rubisco protein. (B) 2-DE gel of total proteins extracted by TCA-A method. (C) 2-DE gel of total proteins extracted by Phe method. (D) 2-DE gel of total proteins extracted by Phe-B method. In all the 2-DE gels, the isoelectric point ranged from 4 to 7. Fourteen pairs of protein spots were randomly selected from the Phe (C) and Phe-B (D) gels for MALDI TOF/TOF (indicated by arrows and numbers). Due to the failure to obtain a clear 2-DE map, no protein spots were selected from the TCA-A gel (B) for MS identification

Back to article page

Plant Methods

ISSN: 1746-4811