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Fig. 5 | Plant Methods

Fig. 5

From: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Fig. 5

Production of rmGM-CSF in the F5 generation of mGM-CSF/OsMYBS2RNAi transgenic rice suspension cell lines. One milliliter of suspension cells, consisting of G5 (mGM-CSF only) and GS2Ri (mGM-CSF/OsMYBS2RNAi), both derived from F3 seeds, and GS2Ri1-2-1-1 and GS2Ri4-1-2-2 (both mGM-CSF/OsMYBS2RNAi), derived from F5 seeds, were cultured in 2 mL sugar-free N6 medium for 5 days. A Samples of culture medium were collected and equal amounts of total medium protein from each sample were subjected to western blot analysis using mGM-CSF antibodies. B The amount of rmGM-CSF was measured in the culture medium by ELISA. Error bars represent the SD from triplicate cultures

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Plant Methods

ISSN: 1746-4811