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Fig. 4 | Plant Methods

Fig. 4

From: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Fig. 4

Recombinant mGM-CSF production profiling in the culture medium of mGM-CSF/OsMYBS2RNAi transgenic rice suspension cell lines. A One milliliter of suspension cells were cultured in 2 mL sugar-free N6 medium for various periods. Samples of the culture medium were collected to determine the concentration of rmGM-CSF by ELISA. Error bars represent the SD from triplicate cultures. B Sample of the culture medium collected at days 5, 7, and 8, and an equal amount of total medium protein from each sample were subjected to western blot analysis using mGM-CSF antibodies. The relative levels of rmGM-CSF abundance were measured using ImageJ software. The rmGM-CSF level was relative to that of G5 suspension cells at day 5, where 1 = equivalent

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Plant Methods

ISSN: 1746-4811