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Fig. 3 | Plant Methods

Fig. 3

From: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Fig. 3

Production of recombinant mGM-CSF in the culture medium of mGM-CSF/OsMYBS2RNAi transgenic rice suspension cell lines. One milliliter of suspension cells, consisting of the wild type (WT), one OsMYBS2 knockdown line S2Ri2, two mGM-CSF gene transgenic lines G3 and G5, and two OsMYBS2 knockdown and mGM-CSF transgenic lines GS2Ri1 and GS2Ri4, were cultured in 2 mL sugar-free N6 medium for 5 days. Samples of the culture medium were collected to determine αAmy3 and rmGM-CSF abundance by western blot analysis with specific antibodies to αAmy3 and mGM-CSF, respectively. Silver staining was used to and visualize bands in the culture medium and represent as the loading control

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Plant Methods

ISSN: 1746-4811