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Fig. 1 | Plant Methods

Fig. 1

From: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Fig. 1

Generation of transgenic rice plants harboring the αAmy3p::mGM-CSF and Ubip::OsMYBS2RNAi chimeric genes. A Schematic representation of the expression cassettes in transgenic rice plants used for a dihybrid cross. For αAmy3p::mGM-CSF, the mGM-CSF cDNA was inserted downstream of the αAmy3 promoter (αAmy3p)–signal peptide (sp) sequence. For Ubip::OsMYBS2RNAi, a 271 bp fragment at the 3′ end of the OsMYBS2 cDNA was ligated in sense and antisense orientations to the GFP cDNA and fused downstream of the Ubi promoter (Ubip). B and C PCR-based genotype detection of the αAmy3p::mGM-CSF and Ubip::OsMYBS2RNAi chimeric genes in F1 progeny (B) and F2 population derived from self-pollinated F1 plants (C). The primer sets for mGM-CSF and OsMYBS2RNAi were used to amplify specifically the αAmy3p::mGM-CSF and Ubip::OsMYBS2RNAi chimeric genes, respectively. Arrowheads indicate the lines GS2Ri1 and GS2Ri4 (OsMYBS2 knockdown and mGM-CSF expression), S2Ri2 and S2Ri6 (OsMYBS2 knockdown only), and G3 and G10 (mGM-CSF expression only), which were selected for further studies

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