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Fig. 8 | Plant Methods

Fig. 8

From: Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica)

Fig. 8

Schematic flew diagram of qPCR genotyping. DNA of loquat leaves was extracted for qPCR genotyping. qPCR genotyping was amplified by allele-specific primers, and the relative copy number of each allele was calculated by 2−ΔΔCt method, and then parameter b [b = a1/(a1 + a2); 0 ≤ b ≤ 1] was calculated to determine the genotype of the tested material. We can select tetraploids of the appropriate genotype as backbone parents according to the breeding goal. More genetic information was obtained by MAS and ploidy analysis

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Plant Methods

ISSN: 1746-4811