High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

Table 2 Protocols analyzed

Protocol number	Pepsin	Gelatinization	Rinsing or neutralization	Cellulase solution (CS)	Amyloglucosidase	Duration of incubation	Temperature of incubation
Protocol number	2 g L⁻¹	80 °C—30 min	Rinsing or neutralization	[] mg L⁻¹	1.5 mL L⁻¹	h	°C
1	P	G	R	1	WA	24	40
2	P	G	N	1	WA	24	40
3	WP	WG	N	8	WA	72	40
4	WP	WG	N	8	A	72	50
5	P	WG	N	1	WA	24	50
6	P	WG	N	1	A	24	50
7	P	WG	N	1	A	72	50
8	P	WG	N	8	WA	72	50
9	P	WG	N	8	A	24	50
10	P	WG	N	8	A	72	50
11	P	WG	N	8	A	72	40
12	P	WG	R	1	WA	24	40
13	P	G	N	1	A	24	50
14	P	G	N	8	WA	72	50
15	P	G	N	8	A	24	50
16	P	G	R	1	WA	24	50
17	P	G	R	1	A	24	50
18	P	G	R	8	WA	24	50
19	P	G	R	8	WA	72	50
20	P	G	R	8	A	72	50
21	P	WG	R	8	WA	24	50

The 21 protocols tested as a combination of the 7 retained parameters: (1) Pepsin concentration at 2 (P) or 0 g.L⁻¹ (WP), (2) presence of gelatinization (G) or absence (WG), (3) rinsing (R) or neutralization (N) steps, (4) concentration of cellulase solution (8 or 1 mg L⁻¹), (5) amyloglucosidase concentration (1.5 (A) or 0 mL L⁻¹ (WA)), (6) 24 or 72 h of incubation time and (7) 50 or 40 °C for incubation temperatures

ISSN: 1746-4811