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Table 2 Protocols analyzed

From: High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

Protocol number Pepsin Gelatinization Rinsing or neutralization Cellulase solution (CS) Amyloglucosidase Duration of incubation Temperature of incubation
2 g L−1 80 °C—30 min [] mg L−1 1.5 mL L−1 h °C
1 P G R 1 WA 24 40
2 P G N 1 WA 24 40
3 WP WG N 8 WA 72 40
4 WP WG N 8 A 72 50
5 P WG N 1 WA 24 50
6 P WG N 1 A 24 50
7 P WG N 1 A 72 50
8 P WG N 8 WA 72 50
9 P WG N 8 A 24 50
10 P WG N 8 A 72 50
11 P WG N 8 A 72 40
12 P WG R 1 WA 24 40
13 P G N 1 A 24 50
14 P G N 8 WA 72 50
15 P G N 8 A 24 50
16 P G R 1 WA 24 50
17 P G R 1 A 24 50
18 P G R 8 WA 24 50
19 P G R 8 WA 72 50
20 P G R 8 A 72 50
21 P WG R 8 WA 24 50
  1. The 21 protocols tested as a combination of the 7 retained parameters: (1) Pepsin concentration at 2 (P) or 0 g.L−1 (WP), (2) presence of gelatinization (G) or absence (WG), (3) rinsing (R) or neutralization (N) steps, (4) concentration of cellulase solution (8 or 1 mg L−1), (5) amyloglucosidase concentration (1.5 (A) or 0 mL L−1 (WA)), (6) 24 or 72 h of incubation time and (7) 50 or 40 °C for incubation temperatures