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Table 1 Reported correlations between in vivo and in vitro digestibilities since 1955.

From: High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

References Authors (year) Forage types Animals feed In vitro protocol step 1 In vitro protocol step 2 Correlation coefficient (R) Prediction equation established
[28] Thurman and Wehunt (1955) SorghumCorn silage Rabbits Solution 1% HCl 15 lb pressure 1 h 0.66
[29] Clark (1958) Thirteen grass species Sheep Rumen jus 0.87
[2] Tilley and Terry (1963) CloverLucerne Sheep Rumen jus HCl 0.1 N2 g L−1 pepsin   y = 0.99x − 1.01
[30] Donefer et al. (1963) Green grass silage Sheep Cellulase + pepsin 24 h 40 °C 0.93
[14] Alexander and McGowan (1966) GrassesLegumesHays Sheep Rumen liquor/buffer mixture HCl–pepsin 0.96 y = 0.97x + 5.05
[31] Deinum and Soest (1969) Hays grassesGrass silages Rumen jus 0.89 y = 1.28x − 44.8
[32] Jones and Hayward (1973) Temperate grasses Sheep Cellulase Trichoderma viride 6.25 g L−1 48 h 40 °C 0.92 y = 0.72x + 32.95
[33] Hartley et al. (1974) GrassesLegumes Sheep NDF [3] Cellulase basidiomycete 16 h 0.96
     Rumen jus [2] 37 °CHCl 0.1 N2 g L−1 pepsin [2] 0.96
[5] Jones and Hayward (1975) Temperate grass Sheep HCl 0.1 N–2 g L−1 pepsin 24 h 40 °C Cellulase “BDH” 48 h 40 °C (Trichoderma viride) 0.96 y = 0.61x + 30.4
[34] McQueen and Van Soest (1975) Temperate grassLegumes Sheep and cattle 45 mg Onozuka cellulase in 6.5 m of 1 M acetate. pH 4.5 40 °C 72 h 70 mg pepsin in 1 mL of 1 M acetate. pH 4.5 40 °C 24 h 0.80
     Rumen jus [2] HCl 0.1 N–2 g L−1 pepsin [2] 0.95
[35] Adegbola and Paladines (1977) Tropical grassesLegumes Sheep HCl 0.05 NPepsin 48 h 40 °C Cellulase Trichoderma (Worthinton Biochemical Corporation) 48 h 40 °C 0.98
[36] Clark and Beard (1977) Green SilageHaysStraw Sheep or cattle HCl 0.1 NPepsin 24 h 40 °C Cellulase (Aspergillus niger) 48 h 40 °C 0.80 y = 39.1 + 0.76x
[37] Dowman and Collins (1977) Grass silage Sheep HCl 0.1 NPepsin 24 h 40 °C Cellulase “BDH” 48 h 40 °C 0.89 y = 0.58x + 31.57
[38] Goto and Minson (1977) Tropical and temperate grasses Sheep HCl 0.1 NPepsin 48 h 40 °C Cellulase “Onozuka SS—P” 48 h 40 °C 0.94 y = 0.69x + 20.3
[39] Roughan and Holland (1977) Temperate grasses and legumes NDF solution Cellulase Trichoderma viride 5 h and 18 h 50 °C 0.98 y = 0.98x − 10.12
[2] Tilley and Terry (1963) Temperate grasses Sheep HCl 0.1 N–2 g L−1 pepsin 24 h 40 °C Cellulase Trichoclerma viride (“BDH”. phosphate citrate buffer. pH 4.6) 48 h 40 °C 0.96 y = 0.56x + 34.7
[40, 41] Kellner and Kirchgessner (1977) Kirchgessner and Kellner (1978) Green silageHaysLegumes HCl 2 N 30 min 100 °C Cellulase (0.1 M Na-acetate buffer. pH 4.5) 24 h 39 °CHCl 0.1 N—pepsin 2 mg L−1 48 h 0.91 y = 1.15x − 12.02
[42] McLeod and Minson (1978) Tropical and temperate grasses HCl 0.1 NPepsin 48 h 39 °C Cellulase “Onozuka SS—P 1500” 48 h 39 °C 0.94 y = 0.70x + 18.2
[43] Adamson and Terry (1980) Hays Sheep HCl 0.1 N2 g L−1 pepsin 24 h 40 °C Cellulase Trichoclerma viride (“BDH”. phosphate citrate buffer. pH 4.6) 48 h 40 °C 0.91 y = 0.68x + 24.18
[6] Aufrere (1982) Green silageHaysLegumes Sheep HCl 0.1 N2 g L−1 pepsin 24 h 40 °C Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.05 M pH 4.6)24 h at 40 °C 0.91 y = 0.793x + 0.125 + ΔForage
[7] Aufrere (1983) HCl 0.1 N2 g L−1 pepsin 24 h 40 °C30 min 80 °C Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.05 M pH 4.6)24 h at 40 °C 0.79
[8] Limagrain (1985) HCl 0.1 N2 g L−1 pepsin 24 h 40 °C30 min 80 °C Cellulase “Onozuka R 10” and amyloglucosidase 24 h 40 °C
[16] Lila et al. (1986) Maize silage Sheep α-Amylase (A-1278 SIGMA) 39 °C 24 hHCl 1 N20 g L−1 pepsin 39 °C 24 h Cellulase (Celluclast 200 L type N de Novo. sodium acetate pH 4.8) 50 °C 58 h 0.25
[44] Aufrere and Michalet-Doreau (1988) Dry forages, dry roughages, energy feeds, protein supplements Sheep HCl 0.1 N2 g L−1 pepsin 24 h 40 °C30 min 80 °C Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.05 M pH 4.6)24 h at 40 °C 0.95 y = 0.699x + 22.6
[45] De Boever et al. (1988) Maize silage Sheep and cattle HCl 0.1 N—2 g L−1 pepsin 24 h 40 °C and 45 min 80 °C Cellulase 24 h 40 °C 0.71 y = 0.610x + 28.2
[15] Barrière et al. (1991) Maize silage hybrids Texels castrated sheep Aufrère [6]Lila et al. [16]Limagrain–EnsitecLibramont–Limagrain (NIRS prediction) 0.540.290.380.37 ––––
[8, 9] Andrieu et al. (1993) Dardenne et al. (1993) Maize silage Sheep Limagrain [8] Limagrain [8] 0.537
     Aufrère [7] Aufrère [7] 0.613
     Lila et al. [16] Lila et al. [16] 0.450
[46] Ferret et al. (1997) Maize silage Sheep Rumen liquor/buffer mixture HCl–pepsin [14] 0.62 y = 0.75x + 0.1728
[11] Aufrere (2007) Green silageSilageHay Sheep HCl 0.1 N2 g L−1 pepsin 24 h 40 °C30 min 80 °C Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.05 M pH 4.6) 0.78 y = 0.630x + 29.7
[17] Damiran et al. (2008) Forages Sheep 28 mL of the McDougall’s solution and 7 mL of ruminal fluid 39 °C 48 h HCl 0.1 N—pepsin 6.6 g L−1 39 °C 48 h(Galyean 1997) 0.58 y = 0.614x + 0.149
[18] Kowalski et al. (2014) Grass silage Sheep Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.01 M pH 4.6) 39 °C 48 h 0.53 y = 0.527x + 0.356
[12] Peyrat (2015) Maize Sheep and cattle Aufrère et al. (2007) Aufrère et al. (2007) 0.736 Y = 37.79x + 0.4936
[13] Virlouvet et al. (2019) Maize HCl 0.1 N24 h 40 °C Cellulase Trichoderma viride “Onozuka R 10” (sodium acetate de 0.05 M pH 4.6)72 h 50 °C