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Table 5 Effects of different culture periods and gametophyte density on sporophyte formation and growth of eastern bracken in ex vitro conditions

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Gametophyte liquid culture No. of sporophytes per pot No. of leaves per plant Leaf length (mm) No. of roots per plant Root length (mm) Shoot FW (mg· plant−1) Root FW (mg· plant−1)
Period
(week)
Gametophyte density (g)
2 0.1 7.3 ± 2.96 dz 3.6 ± 0.29 a 21.3 ± 4.27 a–c 3.4 ± 0.62 a 20.3 ± 3.46 ab 14.0 ± 3.62 ab 2.1 ± 0.45 b
  0.3 4.0 ± 1.00 d 3.4 ± 0.68 a 31.6 ± 11.67 a 4.6 ± 1.52 a 30.4 ± 11.46 a 52.2 ± 37.55 a 9.3 ± 6.64 a
  0.5 6.0 ± 1.00 d 3.3 ± 0.22 a 24.6 ± 2.91 ab 3.3 ± 0.36 a 24.8 ± 5.00 a 19.7 ± 4.55 ab 4.8 ± 1.40 ab
  1.0 5.0 ± 1.53 d 3.4 ± 0.29 a 22.8 ± 6.79 ab 3.2 ± 0.91 a 19.2 ± 2.44 ab 20.3 ± 8.53 ab 4.4 ± 1.47 ab
4 0.1 2.7 ± 0.33 d 3.2 ± 0.59 a 29.1 ± 8.96 a 3.3 ± 1.45 a 20.5 ± 7.02 ab 26.3 ± 15.63 ab 2.0 ± 1.14 b
  0.3 1.0 ± 0.58 d 1.2 ± 0.60 b 9.2 ± 4.59 b-d 0.8 ± 0.44 b 7.9 ± 4.25 bc 2.8 ± 1.44 b 0.2 ± 0.11 b
  0.5 0.3 ± 0.33 d 0.7 ± 0.67 b 4.7 ± 4.73 cd 0.7 ± 0.67 b 3.3 ± 3.26 c 1.4 ± 1.39 b 0.1 ± 0.07 b
  1.0 0.3 ± 0.33 d 0.3 ± 0.33 b 3.9 ± 3.90 d 0.7 ± 0.67 b 2.1 ± 2.05 c 0.5 ± 0.46 b 0.2 ± 0.17 b
6 0.1 115.0 ± 5.20 a 4.2 ± 0.08 a 29.3 ± 0.97 a 4.2 ± 0.08 a 16.1 ± 2.95 a–c 25.4 ± 2.17 ab 2.5 ± 0.50 b
  0.3 85.7 ± 15.19 b 3.9 ± 0.30 a 24.1 ± 0.31 ab 3.4 ± 0.30 a 18.6 ± 0.95 ab 15.6 ± 1.64 ab 1.7 ± 0.22 b
  0.5 56.0 ± 3.61 c 4.1 ± 0.17 a 30.9 ± 1.17 a 4.3 ± 0.14 a 23.6 ± 2.06 ab 28.9 ± 1.15 ab 4.4 ± 0.93 ab
  1.0 67.7 ± 3.18 c 3.7 ± 0.17 a 23.2 ± 1.82 ab 3.5 ± 0.14 a 20.4 ± 1.13 ab 13.0 ± 0.92 ab 1.6 ± 0.13 b
Significance         
Period (A)   *** *** *** *** *** NS *
Density (B)   *** * NS NS NS NS NS
A × B   *** * NS NS NS NS NS
  1. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05. NS, not significant; *, **, and *** indicates significance at P < 0.05, 0.01, and 0.001, respectively. FW, fresh weight