Skip to main content

Table 5 Effects of different culture periods and gametophyte density on sporophyte formation and growth of eastern bracken in ex vitro conditions

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Gametophyte liquid culture

No. of sporophytes per pot

No. of leaves per plant

Leaf length (mm)

No. of roots per plant

Root length (mm)

Shoot FW (mg· plant−1)

Root FW (mg· plant−1)

Period

(week)

Gametophyte density (g)

2

0.1

7.3 ± 2.96 dz

3.6 ± 0.29 a

21.3 ± 4.27 a–c

3.4 ± 0.62 a

20.3 ± 3.46 ab

14.0 ± 3.62 ab

2.1 ± 0.45 b

 

0.3

4.0 ± 1.00 d

3.4 ± 0.68 a

31.6 ± 11.67 a

4.6 ± 1.52 a

30.4 ± 11.46 a

52.2 ± 37.55 a

9.3 ± 6.64 a

 

0.5

6.0 ± 1.00 d

3.3 ± 0.22 a

24.6 ± 2.91 ab

3.3 ± 0.36 a

24.8 ± 5.00 a

19.7 ± 4.55 ab

4.8 ± 1.40 ab

 

1.0

5.0 ± 1.53 d

3.4 ± 0.29 a

22.8 ± 6.79 ab

3.2 ± 0.91 a

19.2 ± 2.44 ab

20.3 ± 8.53 ab

4.4 ± 1.47 ab

4

0.1

2.7 ± 0.33 d

3.2 ± 0.59 a

29.1 ± 8.96 a

3.3 ± 1.45 a

20.5 ± 7.02 ab

26.3 ± 15.63 ab

2.0 ± 1.14 b

 

0.3

1.0 ± 0.58 d

1.2 ± 0.60 b

9.2 ± 4.59 b-d

0.8 ± 0.44 b

7.9 ± 4.25 bc

2.8 ± 1.44 b

0.2 ± 0.11 b

 

0.5

0.3 ± 0.33 d

0.7 ± 0.67 b

4.7 ± 4.73 cd

0.7 ± 0.67 b

3.3 ± 3.26 c

1.4 ± 1.39 b

0.1 ± 0.07 b

 

1.0

0.3 ± 0.33 d

0.3 ± 0.33 b

3.9 ± 3.90 d

0.7 ± 0.67 b

2.1 ± 2.05 c

0.5 ± 0.46 b

0.2 ± 0.17 b

6

0.1

115.0 ± 5.20 a

4.2 ± 0.08 a

29.3 ± 0.97 a

4.2 ± 0.08 a

16.1 ± 2.95 a–c

25.4 ± 2.17 ab

2.5 ± 0.50 b

 

0.3

85.7 ± 15.19 b

3.9 ± 0.30 a

24.1 ± 0.31 ab

3.4 ± 0.30 a

18.6 ± 0.95 ab

15.6 ± 1.64 ab

1.7 ± 0.22 b

 

0.5

56.0 ± 3.61 c

4.1 ± 0.17 a

30.9 ± 1.17 a

4.3 ± 0.14 a

23.6 ± 2.06 ab

28.9 ± 1.15 ab

4.4 ± 0.93 ab

 

1.0

67.7 ± 3.18 c

3.7 ± 0.17 a

23.2 ± 1.82 ab

3.5 ± 0.14 a

20.4 ± 1.13 ab

13.0 ± 0.92 ab

1.6 ± 0.13 b

Significance

        

Period (A)

 

***

***

***

***

***

NS

*

Density (B)

 

***

*

NS

NS

NS

NS

NS

A × B

 

***

*

NS

NS

NS

NS

NS

  1. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05. NS, not significant; *, **, and *** indicates significance at P < 0.05, 0.01, and 0.001, respectively. FW, fresh weight