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Table 4 Effects of different culture periods and gametophyte density on gametophyte morphogenesis of eastern bracken

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Gametophyte liquid culture Morphogenesis of regenerated gametophytes (%)
Period (week) Gametophyte density (g) BG SG S-HG
2 0.1 82.3 ± 3.48 az 10.3 ± 2.88 cd 7.4 ± 3.99 d
  0.3 74.4 ± 3.54 ab 17.4 ± 4.79 b–c 8.2 ± 3.05 d
  0.5 63.6 ± 7.04 a–c 27.7 ± 6.25 a–c 8.7 ± 2.03 d
  1.0 63.2 ± 6.81 a–c 27.8 ± 4.99 a–c 9.1 ± 3.89 d
4 0.1 46.4 ± 16.55 cd 33.4 ± 11.47 ab 20.2 ± 13.82 cd
  0.3 62.3 ± 7.25 a–c 27.6 ± 2.81 a–c 10.0 ± 5.77 d
  0.5 47.6 ± 8.92 b–d 37.1 ± 9.06 a 15.3 ± 2.64 cd
  1.0 49.1 ± 13.1 b–d 30.3 ± 7.97 ab 20.6 ± 5.47 cd
6 0.1 7.1 ± 1.73 e 0.7 ± 0.48 d 92.2 ± 2.19 a
  0.3 28.0 ± 3.72 de 0.0 d 72.0 ± 3.72 b
  0.5 27.4 ± 8.32 de 8.6 ± 4.69 cd 64.0 ± 5.20 b
  1.0 65.4 ± 6.60 a–c 6.4 ± 3.61 d 28.2 ± 4.26 c
Significance     
Period (A)   *** *** ***
Density (B)   NS NS **
A × B   ** NS ***
  1. BG, globular forms of branching gametophyte; SG, spatula gametophyte; S-HG, spatula-heart gametophyte. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05. NS, not significant; **, and *** indicates significance at P < 0.01, and 0.001, respectively