Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Table 4 Effects of different culture periods and gametophyte density on gametophyte morphogenesis of eastern bracken

Gametophyte liquid culture		Morphogenesis of regenerated gametophytes (%)
Period (week)	Gametophyte density (g)	BG	SG	S-HG
2	0.1	82.3 ± 3.48 a^z	10.3 ± 2.88 cd	7.4 ± 3.99 d
	0.3	74.4 ± 3.54 ab	17.4 ± 4.79 b–c	8.2 ± 3.05 d
	0.5	63.6 ± 7.04 a–c	27.7 ± 6.25 a–c	8.7 ± 2.03 d
	1.0	63.2 ± 6.81 a–c	27.8 ± 4.99 a–c	9.1 ± 3.89 d
4	0.1	46.4 ± 16.55 cd	33.4 ± 11.47 ab	20.2 ± 13.82 cd
	0.3	62.3 ± 7.25 a–c	27.6 ± 2.81 a–c	10.0 ± 5.77 d
	0.5	47.6 ± 8.92 b–d	37.1 ± 9.06 a	15.3 ± 2.64 cd
	1.0	49.1 ± 13.1 b–d	30.3 ± 7.97 ab	20.6 ± 5.47 cd
6	0.1	7.1 ± 1.73 e	0.7 ± 0.48 d	92.2 ± 2.19 a
	0.3	28.0 ± 3.72 de	0.0 d	72.0 ± 3.72 b
	0.5	27.4 ± 8.32 de	8.6 ± 4.69 cd	64.0 ± 5.20 b
	1.0	65.4 ± 6.60 a–c	6.4 ± 3.61 d	28.2 ± 4.26 c
Significance
Period (A)		***	***	***
Density (B)		NS	NS	**
A × B		**	NS	***

BG, globular forms of branching gametophyte; SG, spatula gametophyte; S-HG, spatula-heart gametophyte. ^zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05. NS, not significant; **, and *** indicates significance at P < 0.01, and 0.001, respectively

ISSN: 1746-4811