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Table 2 Effects of different culture types on gametophyte morphogenesis of eastern bracken

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Culture types

Morphogenesis of regenerated gametophytes (%)

Total

fresh weight (g)

BG

SG

S-HG

GS

0.0 cz

89.7 ± 2.30 a

10.3 ± 2.30 a-c

6.5 ± 0.32 a

GL

54.5 ± 9.58 b

17.7 ± 2.03 b

27.8 ± 9.03 a

6.9 ± 0.89 a

GL1st

82.4 ± 2.47 a

12.2 ± 2.88 b

5.4 ± 0.87 bc

5.2 ± 0.53 ab

GL2nd

79.5 ± 1.06 a

20.5 ± 1.06 b

0.0 c

5.5 ± 0.58 ab

GL2nd-GS

0.0 c

77.2 ± 9.77 a

22.8 ± 9.77 ab

4.5 ± 0.15 b

  1. GS, gametophyte fragmentation cultured for 6 weeks in solid medium; GL, gametophyte fragmentation cultured for 2 weeks in liquid medium; GL1st, liquid cultured gametophytes (GL) were subcultured and then liquid cultured for 2 weeks; GL2nd, liquid cultured gametophytes (GL1st) were subcultured then liquid cultured for 2 weeks; GL2nd-GS, liquid cultured gametophytes (GL2nd) were subcultured and then solid cultured for 6 weeks; BG, globular forms of branching gametophyte; SG, spatula gametophyte; S-HG, spatula-heart gametophyte
  2. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05
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Plant Methods

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