|
Culture types
|
Morphogenesis of regenerated gametophytes (%)
|
Total
fresh weight (g)
|
|---|
|
BG
|
SG
|
S-HG
|
|---|
|
GS
|
0.0 cz
|
89.7 ± 2.30 a
|
10.3 ± 2.30 a-c
|
6.5 ± 0.32 a
|
|
GL
|
54.5 ± 9.58 b
|
17.7 ± 2.03 b
|
27.8 ± 9.03 a
|
6.9 ± 0.89 a
|
|
GL1st
|
82.4 ± 2.47 a
|
12.2 ± 2.88 b
|
5.4 ± 0.87 bc
|
5.2 ± 0.53 ab
|
|
GL2nd
|
79.5 ± 1.06 a
|
20.5 ± 1.06 b
|
0.0 c
|
5.5 ± 0.58 ab
|
|
GL2nd-GS
|
0.0 c
|
77.2 ± 9.77 a
|
22.8 ± 9.77 ab
|
4.5 ± 0.15 b
|
- GS, gametophyte fragmentation cultured for 6 weeks in solid medium; GL, gametophyte fragmentation cultured for 2 weeks in liquid medium; GL1st, liquid cultured gametophytes (GL) were subcultured and then liquid cultured for 2 weeks; GL2nd, liquid cultured gametophytes (GL1st) were subcultured then liquid cultured for 2 weeks; GL2nd-GS, liquid cultured gametophytes (GL2nd) were subcultured and then solid cultured for 6 weeks; BG, globular forms of branching gametophyte; SG, spatula gametophyte; S-HG, spatula-heart gametophyte
- zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05
