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Table 2 Effects of different culture types on gametophyte morphogenesis of eastern bracken

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Culture types Morphogenesis of regenerated gametophytes (%) Total
fresh weight (g)
BG SG S-HG
GS 0.0 cz 89.7 ± 2.30 a 10.3 ± 2.30 a-c 6.5 ± 0.32 a
GL 54.5 ± 9.58 b 17.7 ± 2.03 b 27.8 ± 9.03 a 6.9 ± 0.89 a
GL1st 82.4 ± 2.47 a 12.2 ± 2.88 b 5.4 ± 0.87 bc 5.2 ± 0.53 ab
GL2nd 79.5 ± 1.06 a 20.5 ± 1.06 b 0.0 c 5.5 ± 0.58 ab
GL2nd-GS 0.0 c 77.2 ± 9.77 a 22.8 ± 9.77 ab 4.5 ± 0.15 b
  1. GS, gametophyte fragmentation cultured for 6 weeks in solid medium; GL, gametophyte fragmentation cultured for 2 weeks in liquid medium; GL1st, liquid cultured gametophytes (GL) were subcultured and then liquid cultured for 2 weeks; GL2nd, liquid cultured gametophytes (GL1st) were subcultured then liquid cultured for 2 weeks; GL2nd-GS, liquid cultured gametophytes (GL2nd) were subcultured and then solid cultured for 6 weeks; BG, globular forms of branching gametophyte; SG, spatula gametophyte; S-HG, spatula-heart gametophyte
  2. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05