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Table 1 Effects of different culture types on sporophyte formation and growth of eastern bracken in ex vitro conditions

From: Culture types and period impact gametophyte morphogenesis and sporophyte formation of eastern bracken

Culture
types
No. of sporophytes per pot No. of leaves per plant Leaf length (mm) No. of roots per plant Root length (mm) Shoot FW (mg· plant−1) Root FW (mg· plant−1)
GS 156.7 ± 16.50 az 3.2 ± 0.29 b 26.4 ± 0.02 b 4.0 ± 0.00 ab 23.2 ± 1.07 a 11.6 ± 0.60 b 2.0 ± 0.19 a
GL 0.8 ± 0.48 b 3.7 ab 38.9 a 5.0 a 20.7 a 67.0 a 7.4 a
GL1st 8.3 ± 4.27 c 3.1 ± 0.22 b 23.3 ± 3.12 b 3.2 ± 0.44 b 22.5 ± 1.75 a 16.5 ± 5.33 b 5.8 ± 2.62 a
GL2nd 8.8 ± 3.09 c 3.5 ± 0.14 b 26.4 ± 0.91 b 4.0 ± 0.00 ab 20.9 ± 1.23 a 19.0 ± 2.66 b 7.3 ± 1.68 a
GL2nd-GS 77.8 ± 5.92 c 4.4 ± 0.22 a 28.7 ± 3.71 b 4.1 ± 0.22 ab 17.4 ± 2.37 a 28.6 ± 7.76 b 2.9 ± 1.07 a
  1. GS, gametophyte fragmentation cultured for 6 weeks in solid medium; GL, gametophyte fragmentation cultured for 2 weeks in liquid medium; GL1st, liquid cultured gametophytes (GL) were subcultured and then liquid cultured for 2 weeks; GL2nd, liquid cultured gametophytes (GL1st) were subcultured and then liquid cultured for 2 weeks; GL2nd-GS, liquid cultured gametophytes (GL2nd) were subcultured and then solid cultured for 6 weeks
  2. zDifferent letters indicate a significant difference using Duncan’s multiple range test at P < 0.05. FW, fresh weight