Table 1 Literature-based comparison of the isolation of protoplasts from various plants
Species | Tissue | Yield (g−1 FW) | Viability (%) | References |
|---|---|---|---|---|
Camellia sinensis (L.) O. Kuntze | Tender leaves | 3.27 × 106 | 92.24 | This study |
Mature leaves | 1.48 × 106 | 83.23 | This study | |
Unlignified branches | 1.20 × 106 | 80.97 | This study | |
Roots | 3.20 × 106 | 89 | [13] | |
First leaves | Description without data | Description without data | [12] | |
Petunia hybrida | Leaves | 1.04 × 106 | 73.3 | [24] |
Ricinus communis L. | Cotyledons and true leaves | 6.1 × 106 | 85 | [25] |
Ananas comosus L. | Leaves | 6.5 × 105 | 51 | [26] |
Fragaria vesca | Leaves | 3.25 × 105 | – | [27] |
Saccharum spontaneum L. | Young leaves | 1.26 × 107 | – | [28] |
Chrysanthemum | 5–8-week-old leaves | 6.32 × 105 | 91.70 | [29] |
Cymbidium | Root | 7.80 × 105 | 89.3 | [4] |
Flower pedicel | 5.26 × 106 | 90.3 | ||
Young leaf | 3.30 × 106 | 91.3 | ||
Leaf base | 2.50 × 107 | 92.1 | ||
Oryza sativa L. | Stem and sheath tissues | 1.00 × 107 | > 95.0 | [30] |
Zea mays L. | Middle parts (6–8 cm) of the second leaves | 1.00–5.00 × 106 | 95.0 | [5] |
Manihot esculenta | Fully expanded leaves | 4.4 × 107 | 92.6 | [31] |
Arabidopsis thaliana | Leaves | 3.0 × 107 | – | [4] |
Malus Pumila Mill | Cell suspension cultures | 5.46 × 106 | 98 | [7] |
Cotyledon | 3.72 × 106 | 80 | ||
Leaves of the axenic shoot culture | 3.57 × 106 | 78 | ||
Leaves from garden | 0.10 × 105 | 32 | ||
Bamboo | Leaves | > 6.67 × 106 | ~ 83% | [32] |
Picea glauca (moench) voss | Cells from 6-day-old subcultures | 4.50 × 106 | 70–90 | [33] |
Pummelo and Tangerine | Leaves | 1.00–3.00 × 106 | 91–96 | [6] |