Fig. 3
From: Leaf infiltration in plant science: old method, new possibilities
Application of leaf infiltration-based methods in plant sciences. Two categories of experiments can be distinguished: 1. The infiltration as an output method in which leaves or their fragments after infiltration are centrifuged in order to recover the apoplastic fluid. The apoplastic fluid can be subjected to further analyses by a wide range of techniques. 2. The use of infiltration as an output method includes experiments in which the application of various substances to the internal leaf tissues is intended to induce the studied effect, such as hormone application or agroinfiltration