Skip to main content
Fig. 7 | Plant Methods

Fig. 7

From: DNA–protein interaction studies: a historical and comparative analysis

Fig. 7

Illustration of the SPR method. It starts by arresting one of the binding partners (ligand) to the surface of a metal surface, followed by injecting the other binding partner (analyte) through a flow channel. A light source emits a single wavelenght light which is directed through a glass prism to the back of the metal surface, being then reflected to a detector with a given angle (reflection θ) and creating a plasmon wave. The binding between the analyte and the ligand causes a shift in the angle at which the light is absorbed (SPR θ). A real-time interaction curve records these oscillations (in resonance units [RU]) as a function of time. Created with BioRender.com

Back to article page