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Fig. 5 | Plant Methods

Fig. 5

From: DNA–protein interaction studies: a historical and comparative analysis

Fig. 5

Representation of the “protective” footprinting technique. This method starts by mixing the nucleic acid of interest with the binding protein under study (on the right). Then, an enzymatic or chemical reagent cleaves the nucleic acid, except for the region protected by the protein, in case a complex is formed between the nucleic acid and the protein. The cleaved nucleic acid is submitted to a denaturing electrophoresis in order to identify the protected segment, represented by a gap in the bands of the restriction products, the footprint, when compared to a lane containing the free DNA cleavage products (control) (on the left). Created with BioRender.com

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