Fig. 3
From: DNA–protein interaction studies: a historical and comparative analysis
Comparison between the cross-linking procedure (a) and South-Western Blotting (b). In the cross-linking tehcnique (a) a nucleic acid is cross-linked to the respective binding proteins, ensuring that all proteins present in the complex are detected in a SDS-PAGE performed after an EMSA (that isolates the complex under study), independently of being in direct contact with the nucleic acid (Protein 1) or not (Protein 2). As for South-Western Blotting (b), after an EMSA, the complex is subjected to a SDS-PAGE without being exposed to a cross-linker and, consequently, only the proteins in direct contact with the nucleic acid will be detected. Created with BioRender.com