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Fig. 1 | Plant Methods

Fig. 1

From: Image analysis for the automatic phenotyping of Orobanche cumana tubercles on sunflower roots

Fig. 1

Phenotyping O. cumana tubercles on inoculated sunflower roots grown in rhizotrons. The susceptible sunflower genotype (2603) was inoculated with the race Bourret (France) of O. cumana in rhizotrons, and the number of tubercles was counted following 3 weeks of culture. a Box containing ten rhizotrons at 3 weeks of culture. b One-day-old plantlet following rhizotron setup. c Inoculated sunflower root system following 3 weeks of culture. d First attachments visible following 8 days of culture (arrowheads). e First tubercles following 15 days of culture. f Buds formed from tubercles following 35 days of culture. Pictures d–f were taken under a binocular microscope. g Distribution of the number of tubercles/rhizotron for 4 independent experiments at 3 weeks of culture. The mean for each experiment is represented by a bar. a–c Bar = 5 cm. d, e bar = 1 mm. f bar = 2 mm

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