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Fig. 3 | Plant Methods

Fig. 3

From: Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean

Fig. 3

The effect of herbicide selection on hairy root growth and transformation frequency. a Hairy root growth status on MS medium with different concentration herbicide selection. Soybean genotype Williams 82 was used here. b GUS staining of transgenic hairy roots after growth on MS medium with different concentration herbicide selection. c Total number of hairy roots per explant on MS medium with different concentration herbicide selection. d Positive hairy root transformation frequency on MS medium with different concentration herbicide selection. Values are mean ± SD of three biological replicates, means with different letters denote a significance difference while similar letters denote no significance (Student’s t-test, P < 0.05). The scale bar indicates 1 cm

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