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Fig. 2 | Plant Methods

Fig. 2

From: Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean

Fig. 2

The effect of inoculation, co-cultivation medium and germination time of seed on hairy root transformation frequency. a Hairy root growth status after inoculated and co-cultivated with 1/2 B5, MS, B5, 1/2 MS medium. The photos were taken at 14 days post inoculation (dpi). Soybean genotype Williams 82 was used here. b, e GUS staining of transgenic hairy roots. c, f Positive hairy root transformation frequency (TF). d Hairy root growth status using cotyledons obtained from 1-, 2-, 3-, 4-, 5-day-germinated (dg) seeds as explants respectively. Soybean genotype Williams 82 was used here. Values are mean ± SD of three biological replicates, means with different letters denote a significance difference while similar letters denote no significance (Student’s t-test, P < 0.05). The scale bar indicates 1 cm

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Plant Methods

ISSN: 1746-4811