A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy

Wang, Maoyao; Li, Xinru; Shen, Yinjuan; Adnan, Muhammad; Mao, Le; Lu, Pan; Hu, Qian; Jiang, Fuhong; Khan, Muhammad Tahir; Deng, Zuhu; Chen, Baoshan; Huang, Jiangfeng; Zhang, Muqing

doi:10.1186/s13007-021-00777-8

Plant Methods

Table 2 Integrative calibration statistics for optimized equations generated for prediction of biomass components in sugarcane stalks

From: A systematic high-throughput phenotyping assay for sugarcane stalk quality characterization by near-infrared spectroscopy

	Calibration								Cross validation
	Rank	N	SCM	Spectrum range (cm⁻¹)	Mean	SD	RMSEC	R²	RMSECV	R²cv	RPD
Dry weight (offline)
Sugars (% dry weigh)
Glucose	15	464	FD	3996–11,988	0.96	0.66	0.15	0.95	0.19	0.92	3.44
Fructose	16	418	FD	3996–11,988	1.70	0.75	0.16	0.96	0.22	0.92	3.44
Reducing sugar	15	457	FD	3996–11,988	2.74	1.48	0.33	0.95	0.42	0.92	3.50
Sucrose	10	394	FD + SNV	3996–11,988	48.93	5.17	1.29	0.94	1.49	0.92	3.48
Total sugar	10	410	FD + SNV	3996–11,988	51.85	5.27	1.32	0.94	1.51	0.92	3.49
Residues	8	410	FD	4759.7–9411.4	48.16	5.34	1.59	0.91	1.68	0.90	3.18
Ratio
Sug/res	10	425	FD	3996–7197.4, 1185.7–11,988	1.08	0.21	0.07	0.90	0.07	0.88	2.94
Suc/total	13	536	SNV	4242.8–8894.5	0.95	0.03	0.01	0.87	0.01	0.83	2.45
Fru/glc	13	427	FD	4104–7251.4, 8825.1–11,185.7	1.95	0.57	0.17	0.92	0.21	0.87	2.75
Dry weight (online)
Sugars
Glucose	19	407	FD	4890.8–11,185.7	0.89	0.49	0.18	0.88	0.21	0.82	2.33
Fructose	12	391	FD	4890.8–7251.4, 8038.3–11,185.7	1.63	0.57	0.27	0.78	0.29	0.74	1.98
Reducing sugar	22	401	FD	4242.8–4767.4, 5785.7–9411.4	2.46	1.02	0.38	0.87	0.44	0.81	2.31
Sucrose	15	514	FD	4890.8–11,972.5	49.52	4.92	1.80	0.87	1.98	0.83	2.49
Total sugar	13	514	FD	4890.8–11,185.7	52.30	5.01	1.84	0.87	1.96	0.85	2.56
Residues	12	495	FD	4890.8–8825.1, 9612–11,972.5	47.52	5.01	1.75	0.88	1.86	0.86	2.69
Ratio
Sug/res	24	473	FD	4104–11,972.5	1.10	0.20	0.06	0.91	0.08	0.86	2.70
Suc/total	18	396	FD + SNV	4752–6834.8, 7344–9411.4	0.95	0.02	0.01	0.86	0.01	0.81	2.27
Fru/glc	31	396	FD	4104–11,972.5	1.94	0.56	0.18	0.91	0.23	0.83	2.41
Fresh weight (online)
Moisture	23	528	FD + SNV	4104–11,972.5	73.21	2.46	0.40	0.97	0.46	0.97	5.40
Sugars
Glucose	34	415	FD	4104–11,972.5	0.25	0.15	0.04	0.93	0.06	0.86	2.66
Fructose	18	405	FD + SNV	4104–11,972.5	0.46	0.18	0.07	0.86	0.08	0.82	2.36
Reducing sugar	24	403	FD	4242.8–5276.6, 5785.7–9411.4	0.67	0.28	0.10	0.88	0.12	0.83	2.39
Sucrose	13	453	FD + SSL	4782.8–11,185.7	13.27	1.91	0.45	0.95	0.49	0.93	3.88
Total sugar	23	514	FD	4104–11,972.5	13.97	2.09	0.50	0.95	0.57	0.93	3.68
Residues	23	459	FD	4104–11,972.5	12.66	1.55	0.43	0.93	0.48	0.90	3.21

N, sample number; SCM, scatter correction methods; SD, standard deviation of reference value; RMSEC, root mean square error of calibration; R², determination coefficient; RMSECV, root mean square err of cross validation; R²cv, determination coefficient of cross validation; RPD, ratio performance deviation; SNV, standard normal variate; SSL, straight line subtraction; FD, first derivative; FD + SNV, a combination of FD and SNV; FD + SSL, a combination of FD and SSL

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ISSN: 1746-4811

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