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Fig. 3 | Plant Methods

Fig. 3

From: A cassava common mosaic virus vector for virus-induced gene silencing in cassava

Fig. 3

Silencing effects and stability of insert size of ChlI gene in the CsCMV-based VIGS vectors. a 5-month-old cassava plants (‘SC10’) were infected with the CsCMV -based VIGS vectors carrying different sizes (133, 236, 345 and 439 bp) of partial ChlI and representative silencing phenotypes by silencing of ChlI were photographed at 35 dpi. b Silencing phenotypes in the different-aged leaves induced by silencing of ChlI using the pCsCMV-ChlI345 at 70 dpi. c RT-qPCR analyses of ChlI mRNA expression in 5-month-old cassava plants infected with the CsCMV-based VIGS vectors carrying different sizes (133, 236, 345 and 439 bp) of partial ChlI. Satistical tests were performed using Student’s t test, compared with the plants infected with non-target control CsCMV-NC (*P < 0.05 and ***P < 0.001). Three independent experiments were performed and each included six plants per treatment group. Error bars indicate the SD. d RT-PCR analyses of the stability of the 345-bp ChlI fragment in pCsCMV-ChlI345 among the different-aged leaves. A total of 12 leaves from the agroinfiltrated leaf to the top-most leaf (L1 to L 12, numbered from the inoculated leaves) were collected for RNA extraction and RT-PCR analysis. The non-target control pCsCMV-NC was used as the control

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