Fig. 2
From: A cassava common mosaic virus vector for virus-induced gene silencing in cassava
Silencing of PDS and ChlI genes in cassava using the CsCMV-based vector. a, Representative silencing phenotypes in 3-week-old cassava (‘SC10’) leaves and stems induced by silencing of PDS or ChlI using the CsCMV-based VIGS vector carrying a fragment of cassava PDS (pCsCMV-PDS487), ChlI (pCsCMV-ChlI345) or control vector (pCsCMV-NC) at 15 and 35 dpi. b Silencing phenotypes in 5-month-old cassava plants (‘SC10’) induced by infection with pCsCMV-PDS487 or pCsCMV-ChlI345 at 35 dpi. c RT-qPCR analyses of PDS and ChlI mRNA expression in 3-week and 5-month-old cassava plants infected with CsCMV-PDS487, CsCMV-ChlI345 and CsCMV-NC. Statistical tests were performed using Student’s t test, compared with plants infected with non-target control CsCMV-NC (**P < 0.01 and ***P < 0.001). Three independent experiments were performed, and each included six plants per treatment group. Error bars indicate the SD