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Table 2 The editing efficiency and mutation type in single-target system and dual-target system

From: A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum

Vector name

Editing efficiency

Heterozygous rate (%)

Homozygous rate (%)

Double allele rate (%)

1300cas9-fw2.2-sgRNA1

95.45

86.36

4.55

4.55

1300cas9-fw2.2-sgRNA2

54.55

27.27

18.18

9.09

fw2.2-sgRNA1 target site

87.5

31.25

12.50

43.75

fw2.2-sgRNA2 target site

43.75

43.75

0.00

0.00

  1. Both 1300cas9-fw2.2-sgRNA1 and 1300cas9-fw2.2-sgRNA2 are single-target vectors; fw2.2-sgRNA1 and fw2.2-sgRNA2 are two different target sites in the dual-target vector