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Fig. 2 | Plant Methods

Fig. 2

From: A highly efficient regeneration, genetic transformation system and induction of targeted mutations using CRISPR/Cas9 in Lycium ruthenicum

Fig. 2

Genome editing in Lycium ruthenicum using CRISPR/Cas9 technology. a fw2.2 gene structure and sequences of the target sites. Black boxes: exons; grey lines: introns; sgRNA target sites and the PAM regions (Red). b The process of transformation (Two-week-old callus; differential shoots after four-week of subculture; elongated shoots after six-week of subculture; eight-week-old rooted transformant). c The combination of large fragment deletions with insertions in a T0 plant. The 1515 bp in italic is the intron. 1281 bp deletions are labeled in gray. 29 bp insertions shown in yellow are almost the inversion of the fragment nearby labeling with green. d Editing type and preference of different target sites

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Plant Methods

ISSN: 1746-4811