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Table 1 Summary of site-specific mutagenesis frequencies in M. sieverii callus

From: Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing

Vector ID Target site Number of callus lines analysed Number of mutated callus lines Mutation frequency (%)
pYL- A 18 13 72.2
B 18 10 55.6
A&B 18 10 55.6
pYL- A 21 14 66.7
C 21 19 90.0
A&C 21 14 66.7
pYL- C&D 23 17 73.9
pYL- A 19 18 94.0
E 19 8 42.0
A&E 19 8 42.1
pTG- A 10 4 40.0
B 10 0 0
A&B 10 0 0
pTG- A 20 9 45.0
C 20 10 50.0
A&C 20 9 45.0
pTG- C&D 5 2 40
pTG- A 14 12 85.7
E 14 3 21.4
A&E 14 3 21.4