Table 1 Summary of site-specific mutagenesis frequencies in M. sieverii callus
From: Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing
Vector ID | Target site | Number of callus lines analysed | Number of mutated callus lines | Mutation frequency (%) |
|---|---|---|---|---|
pYL-â‘ | A | 18 | 13 | 72.2 |
B | 18 | 10 | 55.6 | |
A&B | 18 | 10 | 55.6 | |
pYL-â‘¡ | A | 21 | 14 | 66.7 |
C | 21 | 19 | 90.0 | |
A&C | 21 | 14 | 66.7 | |
pYL-â‘¢ | C&D | 23 | 17 | 73.9 |
pYL-â‘£ | A | 19 | 18 | 94.0 |
E | 19 | 8 | 42.0 | |
A&E | 19 | 8 | 42.1 | |
pTG-â‘ | A | 10 | 4 | 40.0 |
B | 10 | 0 | 0 | |
A&B | 10 | 0 | 0 | |
pTG-â‘¡ | A | 20 | 9 | 45.0 |
C | 20 | 10 | 50.0 | |
A&C | 20 | 9 | 45.0 | |
pTG-â‘¢ | C&D | 5 | 2 | 40 |
pTG-â‘£ | A | 14 | 12 | 85.7 |
E | 14 | 3 | 21.4 | |
A&E | 14 | 3 | 21.4 |