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Table 2 PCR primers used to analyze genomic stability of regenerated in vitro shoots obtained from embryogenic callus cultures of Stipagrostis pennata

From: Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Primer

Primer sequences (5′–3′)

Repeat motif

Allele size range

GenBank accession no

Primer1 (SP10)

F:CGCCTTTGTTGTTTATGAGCAG

(TA)7

165–185

MG978348

R:AGCTAGTGTCCCACGTGTC

Primer2 (SP12)

F:TAGATACGCCGGCTCGTT

GCCC)4

401–420

MG978349

R:GTGATGGCAAGTACGGCAG

Primer3 (SP41)

F:GGAAAGATGCGACAACCCG

(GAA)4

412

MG978355

R:AACTTGAGCAGCCTCTTGG

Primer4 (SP17)

F:ACTGTTGAAACCACGATCCG

(TAA)4

326–350

MG978351

R:GCGGAACATTTGCCTTTGG

Primer5 (SP43)

F:GGCAGAACAAATGGAGCCC

(AAT)4

323

MG978356

R:GCAAACGCATCGAAACCTC

Primer6 (SP23)

F:CTTAGCGCCTGGCCAAATC

(TA)6

297–309

MG978352

R:CCTTTCCTGAAGCTAAACCGAC

Primer7 (SP28)

F:AGGCTCAGTGTCCGCAGAAG

(TC)6

237–243

MG978353

R:AGGCATAGCCAAATGCCAC

Primer8 (SP30)

F:AAAGCGGACGGCATTGTTC

(TA)7

210

MG978354

R:AGAAAGCAAGCTTACGGTGC

Primer9 (SP08)

F:CCGGAAATACAATATCCTACCGC

(CAA)3

288–297

MG968959

R:GTCCGGAGGTCTCTCAAGG

Primer10 (SP15)

F:AGCGTAAAGCTCTCGAGTATG

(TTA)4

413–430

MG978350

R:CGAAGGGAGTCGCAAATTCAC