Fig. 1
From: Rapid identification of mutations caused by fast neutron bombardment in Medicago truncatula
The step-by-step workflow diagram to identify candicate causative deletions for FNB mutants. FNB mutants, either from a segregating population or individual mutant, were sequenced by NGS. The wild type plants, and the wild-type like plants from the same segregating population of FNB mutants were sequenced as well. After the joint variants calling and fitering, deletions were verified by Sanger sequencing, and the causative deletion was identified. The numbers in the brackets represet the genotypes (0/0: homozygous wild type; 0/1: heterozygous; 1/1: homozygous mutant)