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Fig. 3 | Plant Methods

Fig. 3

From: Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus

Fig. 3

Transformation of L. japonicus with pISV-DsRed1 containing effector genes. The effector genes nopL, nopM, nopP and nopT of Sinorhizobium sp. NGR234 were cloned into pISV-DsRed1. A. rhizogenes LBA9402 bacteria carrying the constructed binary vectors were used for transformation. Microscopic analysis of formed hairy roots was performed under bright field conditions (top) and for red fluorescence (RF) emission (bottom) at 28 dpi. Bar = 500 μm. RNA from selected red fluorescent roots was isolated for qRT-PCR analysis to detect effector gene expression (5 plants per RNA extraction). LjUbiquitin was used as a reference gene to normalize the transcript abundance value of a given effector gene. Control plants transformed with pISV-DsRed1 (without effector gene) showed weak background signals in the qRT-PCR analysis. Data indicate means ± SE (n = 3; 3 RNA extractions)

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Plant Methods

ISSN: 1746-4811