Fig. 3

The in vitro infection system in plastic cups discloses root and leaf responses as well as fungal propagation. a Basic set-up of the system: four plants grow on agar medium in the lower plastic cup, while an inverted cup serves as lid. Conidiospores are added in a channel in the middle of the plants (black arrow). A separating plastic layer on the agar medium prevents a direct contact of the leaves with V. longisporum containing agar. Therefore, all fungus detectable in leaves results from spread within the plant. Possibilities for downstream analyses are given. b Relative amount of Vl43 fungal DNA in Arabidopsis WT leaves at the time points indicated. Values of infected samples are given relative to background noise in mock samples (set to 1) (n = 3 each, ± SD). c Representative photos from mock treated and Vl43 infected Arabidopsis WT plants, 12 dpi. d Induction of marker genes in Arabidopsis WT roots at the indicated time points. Values of Vl43 infected samples are given relative to mock samples (set to 1) (n = 3 each, ± SD). e Relative amount of Vl43 fungal DNA as quantified in Arabidopsis leaves: in WT and cyp79b2/b3 at the time points indicated (left) and in WT and ERF105 OE, 12 dpi (right). All values are given relative to WT (set to 1) (n = 3 each, ± SD). f Induction of marker genes in Arabidopsis WT leaves at the indicated time points. Values of Vl43 infected samples are given relative to mock samples (set to 1) (n = 3 each, ± SD). g Relative amount of Vl43 fungal DNA as quantified in Brassica napus WT leaves at the time points indicated. Values of infected samples are given relative to background noise in mock samples (set to 1) (n = 3 each, ± SD). Inlay: cup with B. napus. This Figure statistics: student ‘s t-test relative to mock (d, f) or WT (e), * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001