Fig. 2

The soil-based infection system to study fungal propagation and leaf responses. a Relative amount of Vl43 fungal DNA as quantified in WT leaves at the time points indicated. Values of infected samples are given relative to background noise in mock samples (set to 1) (n = 3 each, ± SD). b Comparison of Vl43 infected and mock treated plants, 21 dpi. c Development of disease symptoms as quantified by green leaf area (upper part) and biomass (fresh weight, lower part) for whole rosettes of mock treated and Vl43 infected WT plants (n = 8 each, ± SD). d Induction of marker genes in Arabidopsis WT leaves at the indicated time points. Values of Vl43 infected samples are given relative to mock samples (set to 1) (n = 3 each, ± SD). e Amount of Vl43 fungal DNA in WT, cyp79b2/b3 and ERF105 OE as quantified in leaves, 21 dpi. Values are given relative to WT (set to 1) (n = 3 each, ± SD). f Analysis of fungal colonization in stems of Arabidopsis, 21 dpi. 100% of the cyp79b2/b3 and 87% of the WT stem segments were severely colonized with V. longisporum (100 stem segments tested in each case). Stem segments of ERF105 OE are less colonized than WT as recently published [13]. g Confirmation of V. longisporum outgrowth by using Vl-sGFP: stem segment with outgrowth (top), enlargement of stem end and outgrowth under fluorescence excitation (middle) and enlargement of the fluorescent hyphae (below). This figure statistics: student ‘s t-test relative to mock (c, d) or WT (e), * p ≤ 0.05, ** p ≤ 0.01