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Table 3 The optimized sporulation protocol tested on Ptr strains and Pyrenophora species resulting in elevated conidia numbers

From: An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis

Species

Isolate

Race

Year Isolated

Location

Crop

Average Spores Per Plate

Traditional Method

Increase

Ptr

M4

1 (ToxA and ToxC)

2009

Meckering (WA)

Wheat

3.00E+06

2.00E+03

1500 X

Ptr

DW5

5 (ToxB)

1998

North Dakota (USA)

Wheat

1.79E+06

1.50E+03

1194 X

Ptr

15 FRG 233

1 (ToxA and ToxC)

2015

Esperance (WA)

Wheat

1.37E+06

  

15 FRG 239

1 (ToxA and ToxC)

2015

Yarrawonga (VIC)

Wheat

3.30E+06

  

15 FRG 022

1 (ToxA and ToxC)

2015

Dandaragan (WA)

Barley

1.75E+06

  

17 FRG 118

1 (ToxA and ToxC)

2017

Wagin (WA)

Wheat

2.47E+06

  

15 FRG 230

1 (ToxA and ToxC)

2015

Cunderdin (WA)

Wheat

3.00E+06

  

17 FRG 135

1 (ToxA and ToxC)

2017

Cowra (NSW)

Wheat

2.64E+06

  

16 FRG 175

1 (ToxA and ToxC)

2016

Wunkar (SA)

Wheat

1.15E+06

  

Ptt

W1-1

"Beecher avirulent"

2009

Wongan Hills (WA)

Barley

1.00E+06

1.00E+03

1000 X

Ptm

SG1

unknown

1996

Badgingarra (WA)

Barley

8.10E+05

5.00E+03

162 X