Fig. 6From: An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentisPtr spore inoculum obtained from the improved protocol remained infectious on wheat. An attached leaf infection assay with viable M4 Ptr spores (1 × 105/ml) demonstrated typical tan spot symptoms (a) whereas the negative controls sprayed with 0.02% Tween 20 solution did not (b)Back to article page