Fig. 2
Components of the cooling device and experimental cooling circuit. The fabricated cooling device (a–c), including the individual device parts (a), view of cap end of the assembled device (b), and view of coolant inlet/outlet end of the assembled device (c). Neoprene-insulated Tygon® tubing, for conducting the chilled ethylene glycol (d), is further wrapped in an additional layer of polyethylene pipe insulation (e). A peristaltic pump (f) delivers the chilled ethylene glycol to the cooling device while situated in the MRI unit (g, h) from the circulating bath (coolant pool) (i) via the insulated tubing. Chilled coolant flows through the sample chamber, containing the sample holder comprised of several individual sample tubes. Sample holders were made to hold tubes of three different sizes (j), to accommodate a range of excised plant specimens