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Fig. 1 | Plant Methods

Fig. 1

From: Experimental comparison of two methods to study barley responses to partial submergence

Fig. 1

Overview of the growth conditions used in this study. a Growth conditions when applying partial submergence with water. Plants were grown for 10 days (at which point, two leaves had emerged). Partial submergence was applied by transferring the pots to a large tub filled with tap water 1 cm above soil level. The plants were kept in the same growth conditions during the duration of the experiment. Control plants were left in the same growth conditions but received normal watering. For the recovery period, plants were taken out of the water and kept in the same growth conditions with a normal watering regime. b Experimental conditions used when partial submergence with a 0.1% starch solution was applied. This protocol was adapted from Mano and Takeda [22]. The plants were grown for 15 days (two visible leaves), after which half of the pots were transferred to a large container filled with a 0.1% (w/v) starch solution, with solution reaching 1 cm above soil level. The plants were kept in the same growth conditions. Control plants were left in the same growth conditions but received normal watering. For the recovery period, plants were taken out of the 0.1% starch solution and kept in the same growth conditions with a normal watering regime. Detailed experimental conditions are provided in Materials and Methods. c Gene expression changes for two core hypoxia response genes (HvADH1 and HvHB) under control conditions (24 h (C)) and after 24 h of partial submergence with either water (24 h (W)) or a 0.1% starch solution (24 h (S)). Average expression relative to that of the reference gene (HvACTIN) is shown (3 to 4 independent replicates were carried out; 3 roots pooled per replicate and conditions). Error bars correspond to standard error of the mean. Significant statistical differences (determined using Student’s t-test) are indicated with: 0.01; * for p-value < 0.05

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